Eppendorf 5402 Manual Updated (2026)
Search the official Eppendorf Technical Support forum using the tag #Eppendorf5402Update or contact their legacy equipment team directly – they still support this legendary centrifuge.
Apply a thin layer of specialized centrifuge lubricant or petroleum jelly to the rotor lid threads and the motor shaft connection point. This prevents seizing.
Utilize the "Soft" mode for sensitive samples to prevent resuspension during acceleration or deceleration. Maintenance and Troubleshooting
Ensure the rotor chamber is clean, dry, and free of debris or ice buildup. Loading and Balancing the Rotor (Critical Safety Step) eppendorf 5402 manual updated
(not the user manual)?
Therefore, the best strategy for obtaining the manual is as follows:
Do not use halogenated hydrocarbons, strong acids, or aggressive alkaline solutions for cleaning. Search the official Eppendorf Technical Support forum using
The is a legacy refrigerated microcentrifuge designed for high-performance separation of temperature-sensitive samples in molecular biology and clinical research. While this specific model has been largely succeeded by newer iterations like the Centrifuge 5420 , maintaining an updated manual and protocol is essential for labs still utilizing this reliable workhorse. Technical Specifications and Capabilities
Adjustable from -4°C to 40°C (some documentation indicates a range down to -9°C).
Wait for the speed display to show zero and the audible notification to sound. Press the OPEN LID button to retrieve your samples. Cleaning and Routine Maintenance Utilize the "Soft" mode for sensitive samples to
| Parameter | Specification | | :--- | :--- | | | 14,000 rpm | | Max. Relative Centrifugal Force (RCF) | 16,000 x g | | Temperature Range | -9 °C to +40 °C (16 °F to 104 °F) | | Timer | 0 to 99 minutes, continuous setting, or momentary "short spin" cycle | | Maximum Density of Liquids | 1.2 g/mL |
Toggle the main power switch located on the side or rear panel of the unit.
Understanding the technical limits of your Eppendorf 5402 ensures safe operation and prevents experimental failures. 14,000 RPM (Rotations Per Minute) Maximum RCF: 16,000 × g (Relative Centrifugal Force) Temperature Range: -9°C to +40°C
The official manufacturer, . The sources for its documentation are manuals and datasheets uploaded by users to third-party sites or hosted on re-seller platforms. Therefore, the information on this page serves as the most "updated" and comprehensive guide available, as it synthesizes all known specifications, typical procedures, and common fixes into a single, practical reference.
The updated manual is not a revision of features but a crucial document for a legacy instrument. While the core functionality remains unchanged, the key "updates" that a user of a second-hand 5402 would look for are: